https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14038 Wed 11 Apr 2018 14:55:16 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14897 R1) signaling within the ventral tegmental area (VTA) alters Fos-protein expression in relapse-relevant regions, including the PVT and the nucleus accumbens shell (NacS). Further, whilst previous findings have shown that blockade of OX_R1 in the VTA prevents reinstatement of Cocaine seeking, we show here that this treatment does not affect natural reward seeking for sweetened condensed milk. In light of the reviewed body of literature, as well as the novel data presented, we discuss the considerations for future pharmacotherapies targeting the orexin and CART systems for relapse prevention.]]> Wed 11 Apr 2018 14:47:04 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14894 Wed 11 Apr 2018 10:51:50 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35731 in-vivo, including in animals with cocaine experience. First, we verified that group III mGluRs regulate orexin cell activity in behaving animals by showing that intra-LHA infusions of the selective agonist L-(+)-2-Amino-4-phosphonobutyric acid (L-AP4) reduces c-fos expression in orexin cells following 24 h food deprivation. Next, we extended these findings to show that intra-LHA L-AP4 infusions reduced discriminative stimulus-driven cocaine-seeking following withdrawal. Importantly, L-AP4 had no effect on lever pressing for sucrose pellets or general motoric behavior. Finally, using whole-cell patch-clamp recordings from identified orexin cells in orexin-GFP transgenic mice, we show enhanced presynaptic drive to orexin cells following 14d withdrawal and that this plasticity can be normalized by L-AP4. Together, these data indicate that activation of group III mGluRs in LHA reduces orexin cell activity in vivo and may be an effective strategy to suppress cocaine-seeking behavior following withdrawal. These effects are likely mediated, at least in part, by normalization of presynaptic plasticity at orexin cells that occurs as a result of cocaine exposure. This article is part of the Special Issue entitled ‘Hypothalamic Control of Homeostasis’.]]> Thu 28 Oct 2021 13:02:35 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36090 Thu 17 Mar 2022 14:39:03 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27474 Thu 13 Sep 2018 16:54:06 AEST ]]>